PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Hashimoto V.Y., Dias J.A., Spohr K.A.H., Silva M.C.P., Andrade M.G.B., Müller E.E. & Freitas, J.C. 2012. [Prevalence and risk factors for Leptospira spp. in cattle herds in the south central region of Paraná state.] Prevalência e fatores de risco associados à Leptospira spp. em rebanhos bovinos da região centro-sul do estado do Paraná. Pesquisa Veterinária Brasileira 32(2):99-105. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid 380, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: freitasj@uel.br The aim of this study was to determine the prevalence of anti-Leptospira spp. antibodies and the risk factors for Leptospira spp. infection in breeding cattle herds in the south central region of Paraná state. It was based on the statistic delineation, serological samples and information regarding the selected farms employed in the study of bovine brucellosis for Paraná state in the context of National Program for Control and Eradication of Brucellosis and Tuberculosis. A total of 1.880 females aged ≥24 months from 274 non vaccinated herds were studied. Serum samples were tested for antibodies against Leptospira spp. using microscopic agglutination test (MAT) with 22 Leptospira serovars. The epidemiological questionnaire was applied on all the selected farms and aimed to obtain epidemiological data. Hundred eighty one of 274 herds were positive for Leptospira spp., presenting prevalence of positive herds of 66.06% (IC95%=60.12-71,65%). Presence of ≥43 cattle (OR=3.120; IC=1.418-6.867), animal purchase (OR=2.010; IC=1.154-3.500), rent of pastures (OR=2.925; IC=1.060-8.068) and presence of maternity paddock (OR=1.981; IC=1,068-3,676) were identified as risk factors for leptospirosis due to any serovar in the multivariate logistic regression. Risk factors for leptospirosis due to serovar Hardjo were presence of ≥43 cattle (OR=3.622; IC=1.512-8,677), animal purchase (OR=3.143; IC=1.557-6.342), rent of pastures (OR=4.070; IC=1.370-12.087) and presence of horses (OR=2.981; IC=1.321-6.726). These results indicate that Leptospira spp. infection is widespread in the south central region of Paraná state and that factors related to the herd characteristic and management are associated with the infection.

• Leptospira spp. leptospirosis leptospirose

Abstract in Portuguese:

RESUMO.- Hashimoto V.Y., Dias J.A., Spohr K.A.H., Silva M.C.P., Andrade M.G.B., Müller E.E. & Freitas, J.C. 2012. [Prevalence and risk factors for Leptospira spp. in cattle herds in the south central region of Paraná state.] Prevalência e fatores de risco associados à Leptospira spp. em rebanhos bovinos da região centro-sul do estado do Paraná. Pesquisa Veterinária Brasileira 32(2):99-105. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid 380, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: freitasj@uel.br O objetivo deste trabalho foi determinar a prevalência de anticorpos anti-Leptospira spp e os fatores de risco associados à infecção em rebanhos bovinos com atividade reprodutiva da região centro-sul do estado do Paraná. Foram utilizados o delineamento estatístico, as amostras sorológicas e as informações referentes às propriedades empregadas no estudo da brucelose bovina no estado do Paraná dentro do Contexto do Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose. Foram estudadas 1.880 fêmeas com idade igual ou superior a 24 meses, provenientes de 274 rebanhos não vacinados contra a Leptospira spp. Para o diagnóstico sorológico da infecção foi utilizada a prova de soroaglutinação microscópica (SAM) com 22 sorovares de Leptospira spp. Em cada propriedade foi aplicado um questionário epidemiológico, a fim de obter informações epidemiológicas e práticas de manejo empregadas. Dos 274 rebanhos analisados, 181 foram considerados positivos para a Leptospira spp., com a prevalência de rebanhos de 66,06% (I.C.95%=60,12-71,65%). Presença de ≥ 43 bovinos (OR=3,120; I.C=1,418-6,867), compra de reprodutores (O.R=2,010; I.C=1,154-3,500), aluguel de pasto (O.R=2,925; I.C=1,060-8,068), presença de piquete de parição (O.R=1,981; I.C=1,068-3,676) foram identificados como fatores de risco para a infecção para qualquer sorovar de Leptospira spp. na análise de regressão logística multivariada. Os fatores de risco para a infecção pelo sorovar Hardjo foram presença de ≥43 bovinos (O.R=3,622; I.C=1,512-8,677), compra de reprodutores (O.R=3,143; I.C=1,557-6,342), aluguel de pasto (O.R=4,070; I.C=1,370-12,087) e presença de eqüinos (O.R=2,981; I.C=1,321-6,726). Estes resultados indicam que a infecção pela Leptospira spp está amplamente distribuída na região centro-sul do estado do Paraná e que fatores elacionados às características das propriedades e ao manejo estão associados à infecção.

Abstract in English:

ABSTRACT.- Silva-Zacarias F.G., Spohr K.A.H., Lima B.A.C., Dias J.A., Müller E.E., Ferreira Neto J.S., Turilli C. & Freitas J.C. 2009. [Prevalence of antibodies against Chlamydophila spp. in herds with bovine abortion of Paraná state, Brazil.] Prevalência de anticorpos anti-Chlamydophila spp. em propriedades rurais com histórico de aborto bovino no estado do Paraná. Pesquisa Veterinária Brasileira 29(3):215-219. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid 380, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: freitasj@uel.br Chlamydophila abortus is a recognized cause of bovine epizootic abortion. Abortion, premature birth and weak lamb/calf, stillbirth and repeat breeding in irregular intervals are the most frequent disease manifestations. The complement fixation test is the recommended by the World Organization of Animal Health (OIE) for Chlamydophila spp. serologic diagnosis. The aim of this study was estimate the prevalence of antibodies against Chlamydophila spp. in cattle herds with abortion, selected inside the sampling design of National Program of Control and Erradication of Brucellosis in Paraná state. Serum samples of 3,102 cows (age ³ 24 months) from 373 herds were analyzed by complement fixation test. Totally, 44 (1.42%) animal were positive with titers ³ 32. The seroprevalence of Chlamydophila spp. in the herds was 8.82% (6.15%-12.17%). Four variables were associated with seroprevalence for Chlamydophila spp. in the final model of logistic regression: confined or semi-confined breeding (OR=3.339, P=0.004), farms with less than 35 cows (OR=3.339, P=0.017), abortion in the pasture (OR=2.372, P=0.037) and pasture rent (OR=3.398, P=0.006) were risk factors for Chlamydophila spp. This bacterium infected a small number of cattle from herds with abortion in Paraná state. Chlamydophila spp impact as abortion cause is reduced in this state.

• Chlamydophila abortus bovine epizootic abortion serology bacteria

Abstract in Portuguese:

ABSTRACT.- Silva-Zacarias F.G., Spohr K.A.H., Lima B.A.C., Dias J.A., Müller E.E., Ferreira Neto J.S., Turilli C. & Freitas J.C. 2009. [Prevalence of antibodies against Chlamydophila spp. in herds with bovine abortion of Paraná state, Brazil.] Prevalência de anticorpos anti-Chlamydophila spp. em propriedades rurais com histórico de aborto bovino no estado do Paraná. Pesquisa Veterinária Brasileira 29(3):215-219. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid 380, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: freitasj@uel.br Chlamydophila abortus is a recognized cause of bovine epizootic abortion. Abortion, premature birth and weak lamb/calf, stillbirth and repeat breeding in irregular intervals are the most frequent disease manifestations. The complement fixation test is the recommended by the World Organization of Animal Health (OIE) for Chlamydophila spp. serologic diagnosis. The aim of this study was estimate the prevalence of antibodies against Chlamydophila spp. in cattle herds with abortion, selected inside the sampling design of National Program of Control and Erradication of Brucellosis in Paraná state. Serum samples of 3,102 cows (age ³ 24 months) from 373 herds were analyzed by complement fixation test. Totally, 44 (1.42%) animal were positive with titers ³ 32. The seroprevalence of Chlamydophila spp. in the herds was 8.82% (6.15%-12.17%). Four variables were associated with seroprevalence for Chlamydophila spp. in the final model of logistic regression: confined or semi-confined breeding (OR=3.339, P=0.004), farms with less than 35 cows (OR=3.339, P=0.017), abortion in the pasture (OR=2.372, P=0.037) and pasture rent (OR=3.398, P=0.006) were risk factors for Chlamydophila spp. This bacterium infected a small number of cattle from herds with abortion in Paraná state. Chlamydophila spp impact as abortion cause is reduced in this state.

Abstract in English:

ABSTRACT.- Dias J.A., Alfieri, A.A., Medici, K.C., Freitas, J.C., Ferreira-Neto J.S. & Müller E.E. 2008. [Risk factors for bovine herpesvirus 1 infection in cattle herds in the West region of Parana State.] Fatores de risco associados à infecção pelo herpesvírus bovino 1 em rebanhos bovinos da região Oeste do Estado do Paraná. Pesquisa Veterinária Brasileira 28(3):161-168. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid 380, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: muller@uel.br The aim of this study was to determine the prevalence of positive herds and to identify the risk factors for BoHV-1 infection in breeding cattle herds in the Western region of Parana State. The statistic delineation, the serum samples and information regarding the selected farms, were the same employed in the study of bovine brucellosis for Parana State in the context of National Program for Control and Eradication of Brucellosis and Tuberculosis. The sampling was performed in two stages. Blood samples were collected from 1930 females aged ³24 months from 295 non vaccinated herds. Serum samples were tested for antibodies against BoHV-1 using an indirect ELISA. The epidemiological questionnaire was applied on all the selected farms and aimed to obtain epidemiological data. Hundred ninety of the 295 herds were positive for BoHV-1, presenting prevalence of positive herds of 64.41% [58.65-69.87%]. The number (³23) of females aged 24 months (OR=2.22; IC: 1.09-4.51), purchased cattle (OR=2.68; IC: 1.48-4.82), use of common grass (OR=5.93; IC: 1.31-26.82), history of abortion in the last 12 months (OR=2.37; IC: 1.09-5.16) and presence of wildlife animals (OR=8.86; IC: 1.11-70.73) were identified as risk factors for BoHV-1 infection in the multivariate logistic regression. These results indicate that BoHV-1 infection is widespread in the studied region and that factors related to the herd characteristic and management are associated with the infection.

• Prevalence epidemiology risk factors bovine herpesvirus 1 indirect ELISA

Abstract in Portuguese:

ABSTRACT.- Dias J.A., Alfieri, A.A., Medici, K.C., Freitas, J.C., Ferreira-Neto J.S. & Müller E.E. 2008. [Risk factors for bovine herpesvirus 1 infection in cattle herds in the West region of Parana State.] Fatores de risco associados à infecção pelo herpesvírus bovino 1 em rebanhos bovinos da região Oeste do Estado do Paraná. Pesquisa Veterinária Brasileira 28(3):161-168. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid 380, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: muller@uel.br The aim of this study was to determine the prevalence of positive herds and to identify the risk factors for BoHV-1 infection in breeding cattle herds in the Western region of Parana State. The statistic delineation, the serum samples and information regarding the selected farms, were the same employed in the study of bovine brucellosis for Parana State in the context of National Program for Control and Eradication of Brucellosis and Tuberculosis. The sampling was performed in two stages. Blood samples were collected from 1930 females aged ³24 months from 295 non vaccinated herds. Serum samples were tested for antibodies against BoHV-1 using an indirect ELISA. The epidemiological questionnaire was applied on all the selected farms and aimed to obtain epidemiological data. Hundred ninety of the 295 herds were positive for BoHV-1, presenting prevalence of positive herds of 64.41% [58.65-69.87%]. The number (³23) of females aged 24 months (OR=2.22; IC: 1.09-4.51), purchased cattle (OR=2.68; IC: 1.48-4.82), use of common grass (OR=5.93; IC: 1.31-26.82), history of abortion in the last 12 months (OR=2.37; IC: 1.09-5.16) and presence of wildlife animals (OR=8.86; IC: 1.11-70.73) were identified as risk factors for BoHV-1 infection in the multivariate logistic regression. These results indicate that BoHV-1 infection is widespread in the studied region and that factors related to the herd characteristic and management are associated with the infection.